Activation of Kraft Lignin by an enzymatic treatment with a versatile peroxidase from Bjerkandera sp. R1.
Identifieur interne : 000C90 ( Main/Exploration ); précédent : 000C89; suivant : 000C91Activation of Kraft Lignin by an enzymatic treatment with a versatile peroxidase from Bjerkandera sp. R1.
Auteurs : R. Taboada-Puig [Espagne] ; T A Lú-Chau ; M T Moreira ; G. Feijoo ; J M LemaSource :
- Applied biochemistry and biotechnology [ 1559-0291 ] ; 2013.
Descripteurs français
- KwdFr :
- MESH :
- enzymologie : Coriolaceae.
- métabolisme : Lignine, Peroxidases.
- Spectroscopie de résonance de spin électronique.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Lignin, Peroxidases.
- enzymology : Coriolaceae.
- Electron Spin Resonance Spectroscopy.
Abstract
Enzymatic lignin activation may be an environmentally friendly alternative to the use of chemicals in the production of wood fibers composites. Most studies on enzymatic activation of lignin for improving the adhesion of lignocellulosic products have been carried out using laccases. In this work, the use of a versatile peroxidase (VP) from the white-rot fungus Bjerkandera sp. (anamorph R1) for activating Kraft lignin was studied. The effect of enzyme dosage, incubation time, and H(2)O(2) addition profile on lignin activation was evaluated by quantifying the phenoxy radicals formed using electron paramagnetic resonance (EPR) spectroscopy. Two alternative enzymatic systems based on the use of VP (a two-stage and an enzymatic cascade system) were also assayed. At optimal conditions (dose of 15 U g(-1) and continuous addition of H(2)O(2) (5.24 μmol h(-1)) during 1 h) the content of phenoxy radicals was doubled as compared with an untreated control. Moreover, using the two-stage VP system, a lignin activation similar to that found at optimal conditions could be reached in a shorter time.
DOI: 10.1007/s12010-012-0023-z
PubMed: 23306879
Affiliations:
Links toward previous steps (curation, corpus...)
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Feijoo</name></author><author><name sortKey="Lema, J M" sort="Lema, J M" uniqKey="Lema J" first="J M" last="Lema">J M Lema</name></author></analytic><series><title level="j">Applied biochemistry and biotechnology</title><idno type="eISSN">1559-0291</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coriolaceae (enzymology)</term><term>Electron Spin Resonance Spectroscopy (MeSH)</term><term>Lignin (metabolism)</term><term>Peroxidases (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Coriolaceae (enzymologie)</term><term>Lignine (métabolisme)</term><term>Peroxidases (métabolisme)</term><term>Spectroscopie de résonance de spin électronique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lignin</term><term>Peroxidases</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Coriolaceae</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Coriolaceae</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Lignine</term><term>Peroxidases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Electron Spin Resonance Spectroscopy</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Spectroscopie de résonance de spin électronique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Enzymatic lignin activation may be an environmentally friendly alternative to the use of chemicals in the production of wood fibers composites. Most studies on enzymatic activation of lignin for improving the adhesion of lignocellulosic products have been carried out using laccases. In this work, the use of a versatile peroxidase (VP) from the white-rot fungus Bjerkandera sp. (anamorph R1) for activating Kraft lignin was studied. The effect of enzyme dosage, incubation time, and H(2)O(2) addition profile on lignin activation was evaluated by quantifying the phenoxy radicals formed using electron paramagnetic resonance (EPR) spectroscopy. Two alternative enzymatic systems based on the use of VP (a two-stage and an enzymatic cascade system) were also assayed. At optimal conditions (dose of 15 U g(-1) and continuous addition of H(2)O(2) (5.24 μmol h(-1)) during 1 h) the content of phenoxy radicals was doubled as compared with an untreated control. Moreover, using the two-stage VP system, a lignin activation similar to that found at optimal conditions could be reached in a shorter time.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23306879</PMID><DateCompleted><Year>2013</Year><Month>08</Month><Day>28</Day></DateCompleted><DateRevised><Year>2013</Year><Month>02</Month><Day>20</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1559-0291</ISSN><JournalIssue CitedMedium="Internet"><Volume>169</Volume><Issue>4</Issue><PubDate><Year>2013</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Applied biochemistry and biotechnology</Title><ISOAbbreviation>Appl Biochem Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Activation of Kraft Lignin by an enzymatic treatment with a versatile peroxidase from Bjerkandera sp. R1.</ArticleTitle><Pagination><MedlinePgn>1262-78</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s12010-012-0023-z</ELocationID><Abstract><AbstractText>Enzymatic lignin activation may be an environmentally friendly alternative to the use of chemicals in the production of wood fibers composites. Most studies on enzymatic activation of lignin for improving the adhesion of lignocellulosic products have been carried out using laccases. In this work, the use of a versatile peroxidase (VP) from the white-rot fungus Bjerkandera sp. (anamorph R1) for activating Kraft lignin was studied. The effect of enzyme dosage, incubation time, and H(2)O(2) addition profile on lignin activation was evaluated by quantifying the phenoxy radicals formed using electron paramagnetic resonance (EPR) spectroscopy. Two alternative enzymatic systems based on the use of VP (a two-stage and an enzymatic cascade system) were also assayed. At optimal conditions (dose of 15 U g(-1) and continuous addition of H(2)O(2) (5.24 μmol h(-1)) during 1 h) the content of phenoxy radicals was doubled as compared with an untreated control. 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Feijoo</name><name sortKey="Lema, J M" sort="Lema, J M" uniqKey="Lema J" first="J M" last="Lema">J M Lema</name><name sortKey="Lu Chau, T A" sort="Lu Chau, T A" uniqKey="Lu Chau T" first="T A" last="Lú-Chau">T A Lú-Chau</name><name sortKey="Moreira, M T" sort="Moreira, M T" uniqKey="Moreira M" first="M T" last="Moreira">M T Moreira</name></noCountry><country name="Espagne"><region name="Galice"><name sortKey="Taboada Puig, R" sort="Taboada Puig, R" uniqKey="Taboada Puig R" first="R" last="Taboada-Puig">R. Taboada-Puig</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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